Uji Antibakteri Ekstrak Kulit Nanas (Ananas comosus (L.) Merr) terhadap Bakteri Eschericia coli
DOI:
https://doi.org/10.59024/jikas.v4i4.2584Keywords:
Antibacterial, Disc Diffusion, Escherichia coli, Inhibition Zone, Pineapple Peel ExtractAbstract
Escherichia coli strains are responsible for digestive issues, which is why natural antibacterial agents are necessary. Several bioactive compounds that have antibacterial properties are present in the waste from pineapple peels (Anas comosus (L.) Merr). The purpose of this research was to assess the antibacterial efficacy of pineapple peel extract against Escherichia coli growth and to compare its effectiveness at different concentrations. It used the disk diffusion technique in a quasi-experimental design. A solution containing 70% ethanol and pineapple peel extract was tested under different conditions: 5%, 10% DMSO as a control, and amoxicillin as the positive control. After incubation, the inhibition zone's diameter was used to measure antibacterial activity. The inhibition zone diameter for 5% concentration was found to be 7.90 mm, while 10% and 15% had an average diameter of 8.17 m. The positive control measured 11.74 h and the negative one recorded 0.39 kg/cm (response vector 1:505-988; response vector 2:253-768). By means of a one-way ANOVA, the treatment groups showed marked differences (F = 301.101; P0.001). The post hoc test conducted by Tukey revealed that the concentrations of 5%, 10% and 15% were all equal to each other.
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